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ISSN (On-line): 1678-9741 Impact Factor: 0.809
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Volume 28 Number 2, April - June, 2013


DOI: http://dx.doi.org/10.5935/1678-9741.20130038

Clinical and ultramicroscopic myocardial randomized study of beating versus arrested heart for mitral surgery

Estudo randomizado clínico e ultramicroscópico na cirurgia valvar mitral com o coração com atividade elétrica versus sem atividade elétrica

Clotario Neptali Carrasco CuevaI; Maiara dos Santos RochaII; Carlos Maurício Cardeal MendesIII; Luiz Antonio Rodrigues de FreitasIV; José Augusto BáuciaV; Roberto BadaróVI

IAdjunct IV Professor of the Medical School of the Universidade Federal da Bahia (UFBA), teacher and cardiovascular surgeon, Salvador, BA, Brazil. Participated in all stages from project design, execution, analysis of results and writing of the article for publication
IIStudent of the Medicine Course of the UFBA, Salvador, BA, Brazil. Participated in the entire project with emphasis on the collection and organization of data
IIIProfessor of Postgraduate Interactive Processes of Organs and Systems/ICS/UFBA, Salvador, BA, Brazil. Performed the statistical analysis of the database
Adjunct Professor of the Medical School of the UFBA, Salvador, BA, Brazil. Senior reseacher of FIOCRUZ. Performed histological analysis with electron ultramicroscopy
Adjunct Professor of the Medical School of the UFBA, Salvador, BA, Brazil, Salvador, BA, Brazil. Participated in the project design, the execution of operations and in the writing
VIProfessor of the Medical School of the UFBA, Salvador, BA, Brazil. Participated in all stages from project design, execution, analysis of results and writing of the article for publication

This study was carried out at Hospital Ana Nery and Centro de Pesquisas Gonçalo Moniz - Fiocruz, Bahia, Salvador, BA, Brazil.

Correspondence To:
Clotario Neptali Carrasco Cueva
Departamento de Anestesiologia e Cirurgia, Disciplina de Cirurgia Torácica e Cardiovascular da Faculdade de Medicina da Universidade Federal da Bahia
Av. Augusto Viana, S/N - Canela
Salvador, BA, Brasil - Zip code: 40110-160
E-mail: calvence@yahoo.com.br


INTRODUCTION: Beating heart surgery on normothermic bypass simulates physiologic cardiac status.
OBJECTIVES: This study compared clinical and transmission electron microscopic aspects of myocardial protection during mitral valve replacement using warm retrograde perfusion in empty beating versus arrested heart with cold blood anterograde cardioplegia.
METHODS: Randomized study to evaluate myocardial cellular ischemia-reperfusion of both techniques to replace the mitral valve. Thirty-four patients were randomly assigned into group A (beating heart) and group B (arrested heart). The following parameters were assessed: echocardiography, blood chemistry, hemoglobin, lactate. During the surgical procedure a total of 102 myocardial biopsies were performed for ultrastructural analysis from anterior left ventricular wall: before cardiopulmonary bypass, before aortic desclamping and 10 minutes after reperfusion.
RESULTS: Elevation of lactate at 3 hours during the procedure was higher in group A, but similar at the end of surgery (P=0.06). Cardioversion was necessary in 5/17 (A) vs. 13/17 (B) P=0.07. Median intraoperative systemic temperature was significantly lower in the group B compared to A (32ºC vs. 36ºC), P<0.001. There was no significant difference of the ultramicroscopic aspects of the heart biopsies before, during and after surgery in both groups. Cellular and mitochondrial transient abnormalities such as mitochondrial swelling, glycogen loss and cytosol swelling were detected independently of the moment of the biopsies.
CONCLUSION: Myocardial protection and ultrastructural abnormalities were similar for both types of mitral valve replacement beating or arrested heart techniques.

Keywords Heart Arrest, Induced. Myocardium. Mitral Valve, Surgery.


INTRODUÇÃO: A cirurgia valvar mitral pode ser realizada com o coração com atividade elétrica, vazio e normotérmico com pinçamento aórtico, perfusão sanguínea no seio coronário, simulando um estado fisiológico.
OBJETIVOS: Comparar as manifestações clínicas e ultramicroscópicas do miocárdio, na cirurgia valvar mitral, com o coração com atividade elétrica versus sem atividade elétrica.
MÉTODOS: Estudo randomizado constituído de 34 pacientes: grupo A (batendo) e grupo B (parado). Os parâmetros foram: hematológico, bioquímico, ecocardiográfico, lactato. Foram realizadas 102 biopsias da parede anterior do ventrículo esquerdo preparadas para análise ultraestrutural: antes da circulação extracorpórea, antes do despinçamento aórtico e 10 minutos após a interrupção da circulação extracorpórea.
RESULTADOS: Verificou-se elevação do lactato 3 horas após o início do procedimento, que foi maior no grupo A (P=0,06), todavia semelhantes no final da cirurgia. A cardioversão foi necessária em (A) 5/17 vs. (B) 13/17, P=0,07. A temperatura intraoperatória média foi significativamente menor no grupo B em relação ao grupo A (32ºC vs. 36ºC), P<0,001. A análise ultramicroscópica das amostras das biopsias do coração antes da circulação extracorpórea, ao término do pinçamento aórtico e após a saída da circulação extracorpórea, revelou anormalidades transitórias semelhantes no citoplasma, núcleos e mitocôndrias em ambos os grupos, independentemente do momento das biopsias.
CONCLUSÃO: A proteção miocárdica na cirurgia valvar mitral apresentou aspectos semelhantes na preservação da integridade ultraestrutural dos cardiomiócitos quando realizada com o coração com ou sem atividade elétrica.

Palavras-Chave Parada Cardíaca Induzida. Miocárdio. Valva Mitral/Cirurgia.


ATC: Activated time coagulation

ATP: Adenosine triphosphate

BMI: Body mass index

CPB: Cardiopulmonary bypass

ECG: Electrocardiogram

FDR: False discovery rates

Fiocruz: Osvaldo Cruz Foundation

ICU: Intensive care unit

KCl: Potassium chloride

LAD: Left anterior descendent artery

MVR: Mitral valve replacement

NYHA: New York Heart Association

TEM: Transmission electron microscopy


During open-heart surgery prevention of ischemia and reperfusion following cardioplegic arrest are essential for myocardial protection [1]. It has been demonstrated that empty beating heart surgery simulates a physiologic cardiac status and is a good method for myocardial protection [2]. On the other hand, most surgeons prefer the conventional hypothermic arrested heart surgery justified by the low risk of air embolism and less blood in the surgical site that improves visualization [3,4]. Several complications are well documented in arrested heart surgery such as increasing the need for inotropic drug support, intra-aortic balloon pumping, prolonging of the length of intensive care, prolonging of hospital stays, increasing cost and mortality rates [5]. Also, low cardiac output syndrome and severe arrhythmias are associated with postoperative morbidity and mortality [6]. Although beating heart valve replacement with continuous coronary sinus perfusion is a good method for myocardial protection because eliminate the use of cardioplegia corollary risk of ischemic reperfusion injury [7,8]. In addition, beating heart surgery produces less cardiac arrhythmia events and no major differences in cerebrovascular events [9,10]. Beating heart technique is being demonstrated to produces less alterations on the biochemistry markers so, have better myocardium protection [11]. The pros and cons for advocating one or the other technique should be taken into consideration to decide between either a beating or arrested heart surgery procedure.

In this report we present the results of clinical and transmission electron microscopy (TEM) myocardial assessment in the immediate and postoperative period in a randomized prospective study to compare mitral valve replacement using arrested versus on-pump empty beating heart surgery.



Design of study

This was a randomized prospective study to compare mitral valve replacement using arrested versus on-pump beating heart surgery. The objective of this study was to evaluate the immediate clinical findings and cellular ischemia and reperfusion injury of both techniques to replace the mitral valve. Herein we will report the clinical and the preliminary cellular and mitochondrial TEM findings of biopsies of the myocardium performed during the surgery heart procedure in both groups.

Local of study

This study was performed at the Hospital Ana Neri, the reference Center for Cardiac surgery of the Federal University of Bahia, Brazil and the transmission electron microscopy studies were performed at Osvaldo Cruz Foundation (Fiocruz), Bahia, Brazil.

Data collection period

The patients were enrolled from April 2010 to March 2011. Demographic, history of dyslipidemia, hypertension personal habits, history of rheumatic fever and other valvulopathies. History of stroke, hematological and laboratory chemistry profiles. Body mass index (BMI) was calculated using the height in relation to weight formula.

Population of study

A total of 34 patients were selected to have mitral valve substitution.

Inclusion criteria: a) all patient included into the study were adults 18-60 years old, b) with an echocardiography diagnosis of mitral and/or tricuspid valve disease due to inflammatory acquired diseases, c) no previous history of cardiac surgery and d) elective indication for valve replacement.

Exclusion criteria: a) with metabolic diseases such as diabetes mellitus and uremia, b) with coronary artery diseases, c) dilated myocardiopathy, d) with severe chronic pulmonary obstructive diseases, e) with present or past history of malignances diseases, f) acute endocarditis, g) with severe pre-operatory laboratory parameters such as creatinine levels >3mg/dL, hemoglobin < 7.0 g/dL, prothrombin time/activity <70% and clotting time > 10 minutes.

Randomization procedure

We used a simple method that consisted in use of a randomization list for the enrollment of the patients: This list was provided by the biostatistician to consecutively enroll into group A or B according to the following random sequence: AAAAAAABBABABBAAABABAABBBBABBBBABB. A Runs Test of validation for randomization was applied Standard Normal = -0.6966, P-value = 0.486; alternative hypothesis: two sided, this test accepted the null hypothesis that this sequence was random. As recommended by CONSORT 2010 [12] a flow diagram was constructed to depict the enrollment of the patients (Figure 1). Surgical risk was measured using EuroScore formula available on web: euroscore.org/calc.htlm



Surgery techniques

Anesthetic protocol was identical for all patients. All procedures were performed with median sternotomy. Prior to aortic and venous cannulation for the institution of CPB, 400 U/kg heparin sulphates was administrated to the patients in order to maintain the activated time coagulation (ATC) values above 480 seconds. During cardiopulmonary bypass (CPB), non-pulsatile flow was kept at 2.0 L/m2/min to 2.5 L/m2/min with a roller pump and hollow-fiber membrane oxygenator were used. Mean arterial pressure during CPB was maintained between 60 mmHg and 70 mmHg. Oxygen flow was maintained during CBP a saturation of 90% concentration.

Group A: All patients received normothermic blood cardioplegia delivered retrograde via coronary sinus [13]. After cross-clamping aorta to avoid air embolism the left heart was kept vented in the ascendant aorta and right pulmonary vein. Surgery was performed under a temperature of 36ºC using an actively warmer system for blood perfusion. Systemic temperature was monitored with a catheter placed into nasopharyngeal. The flow rate was kept between 250-300 mL/min and a peak perfusion pressure of 40-60 mmHg [14]. Myocardial function was monitored continuously during the intraoperative procedure with 5-lead electrocardiogram (ECG). Occurrences of bradycardia or ST-segment changes were considered as sign of myocardial ischemia and were immediately corrected.

Group B: surgery was performed under intentionally actively cooled moderate hypothermia of 32ºC and monitored as same as in group A also a beating alarm sound when threshold limit of 31-34ºC were reached. Myocardial protection was performed using blood cardioplegia 4:1, anterograde, intermittent. With the following components of the solution; for induction: Potassium chloride (KCl) 15 ml, sodium bicarbonate 60 ml, glucose 5% 350 ml, sodium aspartate 13.5 ml, sodium glutamate 13,5 ml. For maintenance: KCl 5 ml, Sodium bicarbonate 60 ml, glucose 5% 350 ml, sodium aspartate 13.5 ml, sodium glutamate 13.5 ml. In all patients, the heart was arrested with 300 ml/min of the induction solution delivered at 4ºC during 2 minute with an interval of 15 minutes into the aortic root at a pressure of 60 mmHg measured directly through a separate port of the cardioplegic cannula. After arrest, low potassium cardioplegia solution (maintenance) was infused via anterograde cannula and the aortic root was perfused through the cannula with oxygenated blood at the same rate as for the induction. Measurement of intramyocadial temperature was not performed. Inotropic support was done using a dopamine solution at the dose of 5 mcg/kg/min when blood pressure was inferior to 50 mmHg.

In both groups, the right atrium was opened longitudinally and another incision was made into the fossa ovale: superiorly toward the superior vena cava and inferiorly behind coronary sinus. Mitral valve replacement (MVR) was performed using a metallic or bioprostheses substitution by interrupted suture. The tricuspid valve repair was done following De Vegas' technique in both groups and the CPB was disconnected [15].

Hemodynamic studies

Echocardiogram was performed before and after the surgery procedure in both groups using a transthoracic technique to measure ejection fraction and the systolic pressure of pulmonary artery. New York Heart Association (NYHA) functional class protocol was adopted to classify the degree of cardiac dysfunction of each patient before and after surgery. Briefly, Class I: cardiac disease, but no symptoms and no limitation in ordinary physical activity, e.g. shortness of breath when walking, climbing stairs. Class II: mild symptoms (mild shortness of breath and/or angina) and slight limitation during ordinary activity. Class III: marked limitation in activity due to symptoms, even during less-than-ordinary activity, e.g. walking short distances (20-100 m). Comfortable only at rest Class IV: severe limitations. Experiences symptoms even while at rest. Mostly bedbound patients.

Myocardial biopsy

During the surgery biopsies were performed as full thickness transmural specimen of three small fragments measuring 0.5 to 15 mm2 from the left ventricle anterior free wall, near to the apex and between distal left anterior descendent artery (LAD) and the diagonal branch. Biopsies were taken with Tru-Cut biopsy needle (Travenol Laboratories Inc.TM) at three different times: (a) before ischemia (b) at the end of ischemia and (c) 10 min after reperfusion. The samples were immediately fixed in cold 2.5% glutaraldehyde diluted in a 0.1-M cacodilate buffer at pH of 7.4. After rinsing in a buffer, fragments were post-fixed in a 1% osmium tetroxide solution mixed with the same buffer during one hour at 4ºC. Fragments were dehydrated with increasing concentration solutions of acetone and then infiltrated with polibed resin. Ultra-fine sections measuring between 50-70 nm were obtained using an automatic ultra-microtome, Leica EM UC7TM and collected with 200-mesh copper grids. Histological semi-thin sections and ultra-structural photomicrographs were performed after samples analysis, registering repetitive patterns. Photomicrographs were obtained using a Zeiss EM-109 TEM operated at 80 KV.

Classification of the microscopic alterations

The assessment of the ultramicroscopic photography's was based of the system grade 0-4. It was observed the mitochondria, cytoplasmic and nuclear changes according to the following criteria: Grade 0: no structural changes, Grade 1: mild intermyofibrillar and intramitochondrial edema, recent chromatic margination and fragmentation and recent lost of glycogen. Grade 2: moderated edema. Mitochondrial swelling, chromatic margination and fragmentation with lost of glycogen. Grade 3: severe edema, sarcolemma bled, myocardial contraction band, mitochondrial amorphous densities, and disruption of sarcolemma membrane. Grade 4: lost of cytoplasmic architecture, myocardial contraction bands, mitochondrial amorphous densities, and disruption of sarcolemma membrane. All analysis of these subcellular ultramicroscopic alteration of the myocytes was done blinded by the pathologist without known which study group were the sample.

Anatomopathology of the replaced valve

All valve that was replaced, immediately after removal was sent to the laboratory for study.

Statistical analysis

A data bank was constructed and analyzed using the R statistical analysis program. Each individual received a code number and the bank was accessed through an individual password. Because this was a randomized trial of superiority hypothesis tests we performed a comparison for proportions using Fisher's exact test. For the comparison of distribution of non-normal quantitative variables between groups we performed the Mann-Whitney test. For quantitative variables with repeated measurement throughout the study we use the analysis of variances. Due to simultaneous analysis of numerous hypotheses we calculated the recommended false discovery rates (FDR) by direct transformation of the P values according to the method proposed by Rosner [16].

Ethical issues

Written informed consent was obtained from each individual patient prior to enrollment into the study. The study was approved by the Ethics Committee of the University Hospital Professor Edgard Santos under the number 098/2009.



Table 1 summarized the preoperative patients profile data obtained in both groups. Of note, the degree of functional classification of the extent of heart failure according NYHA was significantly different between the two groups.


Table 1 - Click to Enlarge


Patients included in the arrested heart group had more severe heart failure. However, heart failure did not correlate with more alteration in the cardiac rhythms. There was no significant difference in cardiac rhythms between the two groups. Also the rate of rheumatic mitral valve disease was found to be similar in both groups.

Intraoperatory aspects

Table 2 summarized all variables that could be compared. Basically, surgery time, CPB and aorta cross clamp time were almost identical. But, the need for defibrillation was marginally significant higher in group B than group A. Also temperature was significant lower in group B as expected because the technique requirement P=0.005). Group A required less use of inotropic drugs during and after surgical procedure than group B since the heart was constantly beating (P=0.005). The most common association procedure was tricuspid annuloplasty (8/17 group A and 14/17 group B), P=0, 07. The median size of the biological or mechanical prosthetic valve was 31 mm in both groups. Only one patient was submitted to comissurotomy mitral valve in group A. Blood loss analysis was confounded by a single patient in group A that had a blood clotting dysfunction and accounted for total blood lost in this group.


Table 2 - Click to Enlarge


The follow up variable analysis are shown in Table 3. There was no difference between both groups for intensive care unit (ICU) time for recovery also occurrence of heart failure independently of the NYHA functional class before and after surgery. Also, the frequencies of arrhythmias were similar in both groups.


Table 3 - Click to Enlarge


Overall, the outcomes of the patients after surgery were similar for both groups. Only one patient died in the arrested heart group ten days after surgery due to septic shock and two in the beating heart group ten and 42 days after surgery due to septic shock and endocarditis respectively. Figure 2 shows the monitoring of hemoglobin values during and immediately after the surgery procedure. As expected the hemoglobin levels in the blood dropped off in both group without significant difference related with the type of surgical procedure. Figure 3 demonstrates the lactate monitoring levels in both groups during the surgery. There was a significant elevation of lactate at 3 hours during the procedure in group A compared with group B, but at the end of the surgery in both groups the levels were similar.





TEM myocardial biopsy findings

Figures 4 (Group A) and 5 (Group B). The figures A, B and C represent the ultramicroscopic aspects of the mitochondria and cytoplasm: figure A (biopsy 1) - before the bypass; figure B (biopsy 2) - at the end of ischemic arrest; and figure C (biopsy 3) - 10 minutes after reperfusion pre, during and post cardiac surgery of the three performed biopsies for both groups respectively. Figures 4A and 5A are biopsies performed before ischemic arrest that demonstrates cytosolic edema with mitochondria swelling grade 1 and glycogen loss seen in the biopsy before ischemic arrest. Figure 4B and 5B are biopsies performed at the end of ischemic arrest that show a sarcoplasmic reticule and mitochondrial swollen with focal cristae disruption grade 1 and 2. Figure 4C and 5C are biopsies performed 10 minutes post CPB, after reperfusion shows the mitochondrial swelling with cristae disruption and condensation, sarcomeres were intact. Counting the frequencies of that alteration was performed a quantitative analysis. In Table 4 we summarized the frequencies of those cellular and mitochondria alterations studied. At the end of ischemic arrest there was an increasing in cellular and mitochondria alterations in both groups. The statistical analysis of the comparison of both groups revealed no significant difference; however, a more detailed description is under preparation and will be subject of another specific publication.


Figure 4 - Click to Enlarge



Figure 5 - Click to Enlarge



Table 4 - Click to Enlarge



In this comparative randomized prospective study of mitral valve replacement using arrested heart versus on-pump empty beating heart surgery we demonstrated that there is no significant difference in terms of clinic aspects between the two surgical procedures independently of the NYHA heart failure classification before and after the surgical intervention. Also, preliminary studies of TEM myocardial biopsies performed pre and post procedure do not favor one method over the other particularly when looking at mitochondria, nuclei or cytoplasmic alterations related to the type of surgical method.

In general, most authors that have compared the techniques beating heart surgery versus the arrested heart for coronary artery bypass graft advocate that the beating heart technique better protects the heart and has better outcomes in terms of duration of mechanical ventilation support, less need of transfusion, less episodes of arrhythmia and lower length of hospitalization. Indeed, less cost is seen with this approach [17-19]. However, fewer are the studies that analyzed the ultrastructural aspects of human cardiomyocites during the surgical procedure. In a systematic review of beating-heart valve surgery, published by Salhiyyah & Taggart [10], 39 reports were reviewed, however only two were randomized control trials. Indeed, the report of Matsumoto et al. [9] that studied 50 patients (25 each group ) concluded that the perfusion technique of either retrograde warm blood or blood perfusion on beating had similar outcome including mortality and complications even though ultra microscopy study were not performed, the biochemical markers of myocardial injury were significantly lower in the retrograde technique. Despite of our study limitation present incomplete biochemistry analysis, were similar for the mortality rates and complications. Otherwise, in the report of Karadeniz et al. [20] that focused on the comparison of neurological parameters for the three groups with a total of 50 patients, even though the beating heart technique did not cross-clamp the aorta to avoid embolism, the results of neurological findings were similar. In our study all 34 patients had none neurological alteration at the immediately post surgery follow up. Also, in the 31 remain patients during 12 moth follow up.

Since 1971 [21] when a warm blood cardioplegia surgery was introduced and routinely used most studies that compared with hypothermic cardioplegia, despite of the majority were observational studies, all of them agree that the major advantage of this technique is that heart beating protects against ischemia reperfusion injury in addition to reduced workload and cardiopulmonary bypass time, special for mitral valve replacement that the repair are done close to physiological conditions in the state of left ventricular beating tonus [10,21,22]. This knowledge has been extrapolated, but not proven by most these studies that beating heart protects against mitochondrial and cellular damage [6,10,23]. Even though our study had the limitation of small number of patients included into the study, the analysis of 2400 microphotographies from 102 biopsies performed among those 34 patients studied patients, do not support the idea of more injury occur in one technique than the other. The preliminary results presented here do not support the idea of beating heart surgery better protects the myocytes of ultrastructure alterations induced by the type of reperfusion, at least in terms of myocardial necrosis, cellular and mitochondrial alterations.

Indeed, suggesting that the myocardial protection for subcellular damages is independent of keep the heart beating or arrested. In the experimental work reported by Kamlot et al. [24] also they reported that dog heart submitted to 180 minutes of cardiopulmonary bypass either with continuous warm or intermittent cold cardioprotection, no difference was noted in the electron microscopy damage with both cardioplegic protections. Interesting, adenosine triphosphate (ATP), creatine phosphate and lactates levels were slightly degraded in both cardioplegia groups, but the adenosine triphosphate levels were significantly reduced in the right ventricular of the warm compared to the cold group. In our study, the time of cardiopulmonary bypass was around 85-95 minutes. Even though we have not analyzed the ATP levels in our study further we will present details of the qualitative and quantitative ultra structural aspects of the myocardium biopsies compared in both groups. As we have presented here, there are no significant differences cellular and mitochondrial alterations between both groups. This is similar to the work of Schaper et al. [1] who reported an ultra-structural morphometric study of myocardial biopsies performed in 31 patients submitted to aortic valve replacement with cardiopulmonary bypass after induction of cardiac arrested surgery which revealed similar results to those we have encountered in our study. Biopsies performed before and after clamping show cellular and mitochondria alteration compatible with ischemia, which apparently are reversible after desclamping.

It has been suggested that the coronary sinus pressure should be maintain 60-80 mmHg when beating heart procedures are selected. Also, the optimal flow rate are not yet determined, it has been suggested that between 150-250ml/min is the minimal necessary to warrant 4-5% of the total cardiac output [6,9]. A limitation of our study was a small number of patients in both groups to careful evaluate this variable. Another important point is the degree of severity of the damage of the valve. Simultaneous antegrade/retrograde warm blood perfusion with a beating heart has been used independent of the severity of valve disease [13]. Even, we used the NYHA class IV cardiac failure score no significant difference was noted using one or another technique. On the other hand studies monitoring troponin I levels monitoring shown no significant difference on the cardiac troponin I concentration of warm reperfusion to cold blood cardioplegia [8,25]. The need of more utilization of dopamine in the arrested group in our study was due to the routine procedure adopted by our anesthesiologist of keep the blood pressure above 50mmHg all time during the surgery. Another limitation of our study was not having done the biopsy of the right ventricle, which could provide better evaluation of this ventricle.



We conclude that no significant cellular and mitochondrial damage occurs with heart surgery for valve replacement using arrested or on-pump empty beating heart surgery. Also, both techniques are safe and have minimal risk of death direct related to the procedure.



We are in debt to Professor Jehorvan Carvalho chief of the Cardiovascular Surgery for his strong support to this work and everybody of the cardiovascular surgery involved in this work. Also, we thank Maria Lucia Vieira for her strong technical support on the Ultramicroscopic tissue sections preparation. We thank Dr. Sanjay Mehta for English revision of the manuscript.



There is no conflict of interest and no participation or approvals from manufactures companies.


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Article received on february 24, 2013.

Article accepted on may 6, 2013.

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